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Fig. 2. Loss of Par-6 and Baz impedes border cell migration. (A-D) Single confocal
section of stage 10 egg chambers stained with anti-Singed antibody (red) or
GFP (green) that are wild type (wt) (A) or contain mosaic clones for a null
mutation in the baz (B-C) or the par-6 (D) locus. Homozygous
mutant cells are labeled by the absence of GFP. o, Oocyte. Scale bar: 50
µm. (E) slbo-Gal4 driver, expressed in the border cells (UAS-GFP)
(green), but not in the polar cells, Fascilin III (red). (F-L) Confocal images
of border cell clusters that are wild type (F,H,J) or that express either two
copies of RNAi par-6 in a par-6 heterozygous null background
(G,K) or two copies of RNAi baz in a baz heterozygous null
background (I,L). RNAi transgenes were activated using slbo-Gal4.
(F-I) Merged confocal z sections of border cell clusters stained with
anti-Singed antibody (red) (F-I) showing either Par-6 (green) (F,G) or Baz
(green) (H,I). The position of the central polar cells (pc) is indicated by
two straight lines. Scale bar: 5 µm. (J-L) Single confocal sections of
stage 10 egg chambers showing Singed (green) and F-actin (red). Scale bar: 50
µm. Arrows indicate border cell cluster.
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