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Fig. 5. Overexpression of Par-6 and Baz in the border cells disrupts polarity. (A-D) Single confocal section of stage 10 egg chambers stained with anti-aPKC. Wild-type egg chambers are shown in A and C. Egg chambers in which Par-6 and Baz are overexpressed in centripetal and posterior follicle cells, which are indicated by the arrows, are shown in B and D. C and D show higher magnification of the cells in A and B. Scale bar: 50 µm. (E-T) Confocal images of border cell clusters, stained for different polarity markers that are wild type (E-H,M-P) or overexpress Par-6 and Baz (I-L,Q-T) using slbo-Gal4. Scale bar: 5 µm. (E-L) Three-dimensional confocal reconstruction of migrating border cells, stained for aPKC (red) and E-cadherin (green). Each panel represents a different view of the same cluster. (M-T) Confocal z sections of border cell clusters of the indicated genotypes: E-cadherin (green) and Singed (red) (M,Q); Sdt (green) and Singed (red) (N,R); ß_ps-integrin (green) and rhodamine phalloidine (red) (O,S); and -tubulin (green) (P,T).

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