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Fig. 3. Relationship between the tip of the DB and the expression domains of
dpp, en and bnl. Anterior is leftwards. Broken lines in
A,B,D indicate the dorsal midline. Tracheal cells are labeled with
lacZ marker of 1-eve-1 tracheal reporter (brown in A, green in B) or
with GFP-moe driven by btl-gal4 (green in C-E). (A) Dorsal view of two
consecutive segments in a stage 15 embryo double-labeled for bnl mRNA
in the epidermis (purple dot) and trachea (brown, outlined in one segment).
Strong expression of bnl mRNA overlaps the tip of the DB, and weaker
expression extends ventrally. (B) Activated MAP kinase expression (red,
arrowhead) detected in the terminal cell. Lateral view. (C) During stage 14,
the tip of the DB (white arrowhead) has entered the en stripe from
behind and localizes immediately ventral to dpp-lacZ expression. (D)
dpp-lacZ expression in dorsal midline at the completion of dorsal
closure. Late stage 15. (E) pMAD expression (purple) in the DB (green) of a
stage 14 embryo (white arrowhead). A grayscale image of pMAD expression is
shown on the right.
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