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Fig. 1. The moe leaf phenotype. (A) Short-day grown plants of clf-50,
emf2-10 and wild-type progenitor (Ws ecotype) after 30 days. The
emf2-10 plant is slightly earlier flowering and smaller than clf-50.
(B) Fifth rosette leaf of long-day grown plants. The emf2-10 leaf is
smaller and narrower than clf-50, but has similar upward curling of
leaf margin. (C) Seedlings showing effects of emf2-10 on cotyledon
size. (D) Wild-type and emf2-10 flowers at anthesis. (E)
emf2-10 flowers showing delayed flower opening (left) resulting in
contorted siliques (right). (F) emf2-10 flowers from apex of
inflorescence. Arrowheads indicate carpelloid sepals; petals are also
stamenoid in shape and have yellow anther-like sectors. Flowers appear
terminal because flower buds from shoot apex (between two flowers) aborted
early in development and are no longer visible. (G-M) Transgenic plants
carrying AG or AP3 reporter genes stained for GUS activity
(blue colour). (G) pAG-I::GUS activity in clf-2 seedlings.
Expression was also seen in cotyledons at earlier stages. (H)
pAG-I::GUS in emf2-10 seedling. (I) pAG-I::GUS in
wild-type inflorescence. (J) pAG-I::GUS in clf-2
inflorescence. Arrow indicates expression in the stem. (K) pAG-I::GUS
in emf2-10 plant. Arrow indicates expression in inflorescence stem.
(L) pAP3::GUS in clf2 seedling. (M) pAP3::GUS in
emf2-10 seedling. (N) The emf2-10 ag-2 double mutant (arrow)
is shown between ag-2 and emf2-10 parent lines. Note that
the double mutant is earlier flowering than ag-2 and smaller. (O) The
emf2-10/emf2-3 heterozygote (arrow) is shown between emf2-10
and emf2-3 parents. Its phenotype is intermediate, both with respect
to height and overall plant size. Scale bar: 5 mm in A,B; 2 mm in C-O.
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