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Fig. 2. Scanning electron microscopy of Pc-G mutants. (A) Wild-type leaf, abaxial
epidermis. (B) emf2-10 leaf, abaxial epidermis. The surface is
extremely uneven compared with wild type. (C) Wild-type fifth rosette leaf.
The leaf was frozen and fractured to reveal internal anatomy transverse to
leaf length axis. The leaf is patterned along dorsal-ventral axis into
epidermal cells (e), a palisade mesophyll layer (p), and a spongy mesophyll
(s) (D) Fifth rosette leaf of emf2-10 plant of similar age. A similar
arrangement of cell types is seen as in wild type, but the cells are smaller.
(E) emf2-10 flower showing contorted silique. (F) Wild-type flower
showing abaxial epidermi of sepals (se) and petals (pe). The sepal epidermis
contains characteristic highly elongated cells (arrow); however, the margin
lacks the elongated cells and has smaller, more regularly sized cells
(arrowhead). (G) emf2-10 sepal, elongated cells extend to the margin.
(H) emf2-10 flower showing carpelloid sepal. The organ has elongated
cells typical of sepals but stigmatic papillae (arrowhead) and stylar cells
(arrow) characteristic of carpels. (I) Wild-type petal, abaxial surface, note
lack of stomates. (J) emf2-10 petal, abaxial surface. Note presence
of stomates (arrow) and cell shape, characteristic of stamen epidermis. (K)
swn-1 clf-50 inflorescence. The sepals show weak homeotic conversion
to carpelloid organs. Arrowheads indicate stigmatic papillae. (L) Radialised
organ (arrow) with stigmatic papillae arising from inflorescence stem of
swn-1 clf-50 double mutant in position where stipule would normally
arise. (M) swn-3 clf-50 double mutant. Organs arise with disorganised
phyllotaxy. Note lack of trichomes, cells lack wall thickening and are
isodiametric. (N) swn-2 clf-50 plant showing radialised organs. Scale
bar: 100 µm throughout.
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