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Fig. 5. Interaction between Arabidopsis CLF and EMF2 homologues in yeast.
Two-hybrid constructs were introduced into the yeast strain AH109, which
contains reporter genes that confer histidine and adenine prototrophy. The
ADE3 reporter is extremely stringent. Three independent transformants were
stamped onto –LW and –LWHA media. –LW selects for markers on
the bait and prey constructs, while –LWHA also selects for activation of
the HIS3 and ADE2 reporters. (A) Interaction of the VEFS
domain of FIS2 (residues 466-692) with full-length MEA protein. (B) Cladogram
of plant E(z) homologues. Drosophila E(z) is included as an outlier.
Analysis was performed using the PAUP program to align the SET domains of the
proteins. The bootstrap values are indicated. (C) Interaction of SWN C5 domain
(252-331) with the VEFS domains of EMF2 (510-631), VRN2 (275-440) and FIS2
(394-692). (D) Interaction of CLF C5 domain (257-331) with the VEFS domains of
EMF2, VRN2 and FIS2.
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