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Fig. 6. Pluripotent stem cells and their ectoderm derivatives exhibit laminin
receptors. (A-D) Confocal images. (A) Day 4, 1C6 dnFgfr ES cells activated
with Lamc1+/- GATA6 culture supernatant. Stained with
antibody to laminin 
1. The single layer of columnar cells covered by
laminin 1 (red). (B) 1C6 dnFgfr ES cells incubated with
Lamc1-/- GATA6 culture supernatant harvested between day
four and day five of culture and stained with antibody to laminin 1.
Differentiation and laminin 1 staining are both absent. (C) Wild-type
ROSA11 ES cells on feeder layer stained with antibody to laminin 1 and
phalloidin (green). See fibroblast in left upper quarter. There is a lack of
laminin staining. (D) Wild-type ROSA11 ES cells on feeder layer treated with
ROSA11-GATA6 culture supernatant for 6 hours and stained with antibody to
laminin 1 (red) and phalloidin (green). There is laminin deposition in
the membrane of all ES cells.
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