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Fig. 4. Lamina glial cell migration occurs normally in otk3
mutants. Third-instar eye–brain complexes were double-stained with
anti-Repo (red) and anti-ß-galactosidase antibody (green).
Anti-ß-galactosidase antibody was used to visualize all R-cell axons in
both wild type and otk3 mosaic individuals that carry a
glass-lacZ transgene, in which the expression of lacZ is
under control of the eye-specific glass promoter
(Mismer and Rubin, 1987).
Anti-Repo recognizes the nuclear protein Repo expressed in all types of glial
cells. In wild type (A), all R1-R6 growth cones (green) stop in between two
layers of glial cells (red), epithelial (eg) and marginal glia (mg), forming
the lamina plexus (lp). In an otk3 mosaic individual (B),
lamina glial cells were still present at the R1-R6 termination site. Scale
bar: 20 µm.
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