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Fig. 6. The completed pattern of R1-R6 connectivity was severely disrupted in adult
otk mutants. Cryostat sections of wild-type (A,C) and
otk3 eye-specific mosaic heads (B,D) were stained with MAb
24B10 to visualize all R-cell axons. In wild type (A), R7 and R8 axons
projected into different layers within the medulla. Individual R7 and R8 axon
terminals could be readily identified. In an otk3 mosaic
head (B), the medulla appeared to be innervated by an increased number of
R-cell axons. C and D are higher magnification views of the boxed region in A
and B, respectively. Whole-mount wild-type (E) and otk3
mosaic brains (F) carrying an adult R1-R6 specific marker, Rh1-LacZ,
were stained with anti-ß-galactosidase antibody. In wild type (E),
LacZ staining was exclusively observed in the lamina, as all R1-R6
axons terminate within this layer. In an otk3 mosaic brain
(F), a large number of R1-R6 axons (arrow) connected abnormally to the
medulla. Scale bar: 20 µm in A,B,E,F; 5 µm in C,D.
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