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Fig. 1. Expression of the testis TAF homologs nht, rye, mia and
sa. (A) Northern blot of poly(A)+ selected RNA probed
sequentially with sequences from the protein coding regions of nht,
rye and mia, and with rp49 as loading control. (B)
RT-PCR products amplified using primer pairs for sa, its generally
expressed Drosophila homolog dTAF8, and rp49 as
loading control from Poly(A)+ RNA. In both A and B, RNA was from:
wild-type adult males (lane 1), wild-type adult females (lane 2), adult males
lacking germline (lane 3), 0- to 24-hour embryos (lane 4). (C-F) nht, rye,
sa and mia mRNAs were expressed in primary spermatocytes. In
situ hybridization to wild-type whole adult Drosophila testis with
single stranded antisense riboprobes: (C) nht, (D) rye, (E)
sa, or (F) mia. Arrows indicate the onset of mRNA expression
at the start of the primary spermatocyte stage.
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