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Fig. 2. Expression pattern of Cv-2 in the chick embryo. Whole-mount in situ
hybridisations of Cv-2 (A-F) and Slug (K-N) and transverse sections of Cv-2
hybridised embryos (G-J). (A) HH stage 9– (seven-somite stage). Cv-2
expression is detected in posterior lateral mesoderm (lm) and in premigratory
neural crest (nc) in the hindbrain and anterior spinal cord. (B) Stage 9
(eight somites). Cv-2 expression is upregulated in premigratory neural crest
in the hindbrain and spinal cord, and in some cells in the midbrain. (C) Stage
9+ (10 somites). Expression seen at stage 9 persists but (D) by stage 10+ (13
somites) Cv-2 expression in the hindbrain and anterior spinal cord is
downregulated. Low levels of Cv-2 expression occur in the hindbrain in
rhombomeres 3 and 5. (E) Stage 13. Cv-2 transcripts are detected at the
posterior lip of the otic vesicle (ov), the outflow tract of the heart (ot)
and sclerotome surrounding the dorsal aorta between the branchial arches (sc).
(F) Stage 19. Cv-2 transcripts are detected in the intersomitic region (is),
in sympathetic ganglia (sg), in the outflow tract of the heart (ot) and in
nephrogenic mesenchyme (nm). (G,H) Transverse sections through the neural tube
of stage 9+ as indicted in C show that Cv-2 transcripts are restricted to
premigratory neural crest cells (nc) in the dorsal neural tube. (I,J)
Transverse sections through a stage 19 embryo, showing Cv-2 expression in (I)
sympathetic ganglia (sg) adjacent to the dorsal aorta (da) and in (J)
nephrogenic mesenchyme (nm) surrounding the nephric ducts. (K-N) In situ
hybridisation to detect slug transcripts, a marker of premigratory and
migrating neural crest from stage 9– to stage 10+. Black arrowheads
identify the midbrain/hindbrain boundary, black arrows indicate the posterior
limit of the hindbrain.
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