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Fig. 5. Effect of elevated Cv-2 expression on trunk neural crest cell migration.
(A-E) Representative images of the trunk region of stage 16 chick embryos
electroporated with pCIG (A,B) or co-electroporated with p
CS2Cv-2 and
pCIG (C-E) expression constructs at HH stage 10+. (A) In situ hybridisation
with Sox10 of a control pCIG electroporated embryo. (B) Transverse section of
embryo (A) at axial level indicated; black and white arrows identify equal
progression of Sox10-positive migratory neural crest cells on control and
electroporated sides. (C) In situ hybridisation with Sox10 of an embryo
overexpressing Cv-2. The domain of Sox10 expression extends further caudally
on the side overexpressing Cv-2 (right) than the control side (left). Insets
in A and C show the distribution of GFP-positive cells, demonstrating the
domain and efficacy of the electroporation prior to in situ hybridisation
analysis; left side of the neural tube is the control; right side is the
electroporated side. (D,E) Transverse sections through embryo (in C) as
indicated by lines, with immunofluorescence labelling of HNK1.
(D',E') Corresponding bright-field images in which the Sox10
signal is more easily seen. In the more anterior section (D,D') there is
an increase in the number and distance of migration of HNK-1-positive
migratory crest cells on the transfected side (black arrow) compared with the
control side (white arrow). Sox10 signal is not detected in the ventral
HNK1-expressing neural crest, perhaps owing to a decreased level of expression
during ventral migration. In the more posterior section (E,E') Sox10-
and HNK-1-positive crest cells have initiated migration on the Cv-2
electroporated side (black arrow) but not on the untreated side.
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