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Fig. 2. No expression of the zone-specific genes Nqo1 and Ncam2 in OE of Lhx2^-/- embryos. (A) Autoradiographs of in-situ hybridization analyses of consecutive coronal sections (dorsal up and medial left) of one nasal cavity of control embryos (A-C) and Lhx2^-/- embryos (D-F). Sections were hybridized with ³⁵S-labeled cRNA probes specific for Lhx2 and mutated Lhx2 transcripts (Lhx2^m) (A,D), Nqo1 (B,E) and Ncam2 (C,F). Lhx2 expression is distributed throughout the OE in both control (A) and Lhx2^-/- mice (D). Nqo1 is expressed in OSNs located in the dorsomedial zone in control embryos (arrow in B), whereas no signal is present in the neuronal cell layer of Lhx2^-/- embryos (E). Ncam2 is expressed in OSNs located in the middle and the ventrolateral zones in control embryos (arrow in C), whereas no hybridizing signal is present in the neuronal cell layer of Lhx2^-/- embryos (F). The border between neurons in the dorsomedial zone and the middle zone is indicated (dotted line in B,C). Nqo1-specific hybridization to cells in lamina propria (arrowheads in B,E) and Ncam2-specific hybridization to cells in the telencephalon (arrowhead in F) are also indicated. (B) High-power magnifications of OE sections hybridized with Dig-labeled cRNA probes specific for Nqo1 (A,C) and Ncam2 (B,D) are shown. Both probes hybridize to OSN in control embryos (A,B), whereas no signal over background is evident in sections of Lhx2^-/- embryos (C,D).

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