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Fig. 4. The neuronal cell layer in OE of Lhx2-/- embryos
largely contains newly differentiated neurons that have acquired pan-neuronal
traits and form axon bundles. Coronal sections (dorsal up and medial left) of
one nasal cavity from control embryos (A,C,E,G,I,K,M,O) and
Lhx2-/- embryos (B,D,F,H,J,L,N,P). (A-F)
Immunohistochemical analyses for expression of immature neuronal markers is
shown. Ncam1 (A,B), Gap43 (c-d), and
Stathmin/SCG10 (E,F) are expressed in OE and axon bundles of
the lamina propria (arrows) in both control and Lhx2-/-
embryos. (G,H) Double phospho-H3 (PH3; in green) immunohistochemistry and
Neurod1 (ND; in red) in-situ hybridization analyses showing that the
increased expression of Neurod1, primarily apparent in the ventral OE
(arrowhead in H), does not correlate with the number and distribution of
mitotic cells. (I-L) Analyses of consecutive OE sections, showing that cells
immunoreactive using an anti-TubIII antibody (I,J) and cells that express
Neurod1 transcripts (K,L) co-localize predominantly in ventrolateral
regions of OE (arrowheads in J,L). Expression of TubIII in axon bundles of the
lamina propria is indicated (arrows in I,J). (M,N) High magnification confocal
images of double TubIII immunohistochemical (in green) and Neurod1
in-situ hybridization (in red) analyses showing an increased number of cells
in Lhx2-/- embryos that co-express Neurod1 and
TubIII (yellow signal; insert in N). (O,P) High magnification confocal images
of double phospho-H3 immunohistochemical (in green) and Neurod1
in-situ hybridization (in red) analyses showing that OE in
Lhx2-/- embryos does not contain an increased number of
progenitors that co-express phospho-H3 and Neurod1 (in yellow;
arrowheads).
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