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Fig. 2. BMP-dependent NC delamination from explanted neural primordia is inhibited by the G1/S transition blocker mimosine. (A) Control, and (B) 600 µm mimosine, (C) 100 ng/ml BMP4 and (D) mimosine+BMP4 treatment. Left panels are phase-contrast images and right panels depict BrdU immunoreactivity. (E) Data quantification. NC delamination is significantly stimulated by BMP4 (P<0.005) and inhibited by mimosine (P<0.005) relative to controls. Mimosine prevented BMP4-induced NC delamination (P<0.002 relative to BMP4 alone) and BrdU incorporation. Scale bar: 75 µm.





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