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Fig. 1. BRL genes encode homologs of BRI1. (A) Phylogenetic tree of members of the
BRI1-family. Full-length protein sequences were aligned with ClustalX and
manually adjusted. The N-J tree was calculated with MEGA2.1
(Kumar et al., 2001) for 1000
bootstrap repetitions. Branch lengths are proportional to the number of
substitutions per 100 residues (indicated by the bar below the tree) and the
branchpoint values indicate the percentage bootstrap support. The comparison
included the Arabidopsis family members BRI1 [AtBRI1
(Li and Chory, 1997),
AAC49810], AtBRL1 (NP_175957), AtBRL2
[(Clay and Nelson 2001),
NP_178304], AtBRL3 (NP_187946), the corresponding sequences from rice, OsBRI1
[(Yamamuro et al., 2000),
BAB68053], OsBRL1 (BAD01717) and OsBRL2 (AAK52544), and those reported for
tomato [LeBRI1, cu3 (Montoya et
al., 2002), Q8GUQ5], pea [PsBRI1, lka
(Nomura et al., 2003), Q8GUQ5]
and barley [HvBRI1, uzu (Chono et
al., 2003), BAD06331]. Arabidopsis CLV1 (Q9SYQ8) was
included as an outgroup RLK. The Arabidopsis proteins are highlighted
in blue. (B) Diagram of the BRI1 and BRL proteins showing the location of the
T-DNA insertions. Like BRI1, no introns are present in any of the
BRL genes. (C) Alignment of the Arabidopsis proteins. In the
extracellular region, the LRR domain is underlined in red, the arrowheads
indicate the beginning of each LRR, the 70-amino acid island domain region is
underlined in purple and the paired cysteines flanking the LRR-domain are
indicated by black dots. The Ser/Thr kinase domain is underlined in black.
Identical and similar amino acids are highlighted by black and gray boxes,
respectively.
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