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Fig. 6. Notch activation in Delta and lqf-null eye disc clones. Confocal images of eye discs in the region of the furrow are shown. Anterior is upwards in all panels. Eye discs are labeled with mAb323, which recognizes E(spl) proteins. (A,A') An eye disc containing a Delta^rev10 clone marked by the absence of GFP is shown. In A, the clone is outlined and the asterisks indicate Delta^rev10 cells that express E(spl). (B,B') An eye disc containing a lqf ^ARI clone marked by the absence of GFP is shown. In B, the clone is outlined and the asterisks indicate lqf ^ARI cells that express E(spl). The clones were examined throughout the depth of the eye disc and most E(spl)-expressing cells are adjacent to clone borders at all levels. Some E(spl)-positive cells are several distances from the clone border (as in A,A'). This may be evidence for long-range Delta signaling, a process that is not well understood (De Joussineau et al., 2003). Scale bar: 10 µm.

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