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Fig. 1. Phenotypic effects of hyperactivated EGL-15. (A,B) ccIs4251 [P_myo-3::GFP]; soc-2(n1774) animals are egg-laying proficient, and animals display a normal number of embryos in the uterus. (C,D) soc-2(n1774); ayIs15 [egl-15(neu^*); P_myo-3::GFP] animals display a severe Egl phenotype, with an increased number of eggs retained in the uterus. (E,F) soc-2 + / + mIs11; ayIs15/+. EGL-15 remains hyperactivated in these integrated lines as indicated by the dominant Clr phenotype revealed when the recessive soc-2 suppressing mutation is heterozygous. ccIs4251/+; soc-2 + / + mIs11 animals do not display a Clr phenotype (data not shown). mIs11 [P_myo-2::GFP] was used to identify heterozygous cross progeny. All animals were photographed 30 hours after late L4. Scale bar: 100 µm in A,C,E; 50 µm in B,D,E. ^*, eggs. Arrow indicates clear fluid in the pseudocoelomic space.

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