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Fig. 4. Visualization of sex muscles with {alpha}-EGL-15 antibodies. (A,B) ccIs4251; ayIs29 [egl-15(+)] animals display wild-type sex muscle morphology and positions. EGL-15 expression is restricted to the vm1s in differentiated hermaphrodite sex muscles. (C,D) SM descendants in soc-2; ayIs15 animals. Red, {alpha}-EGL-15 immunoreactivity; green, muscle nuclei visualized using Pmyo-3::GFP transgenic arrays. A,B, ventral view; C,D, lateral view. All animals are positioned with the tail oriented towards the right. Arrowheads indicate the position of the vulva. Scale bar: 50 µm in A,C; 10 µm B,D.





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