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Fig. 2. Spatial and temporal expression of Bcre-32 using the ROSA reporter demonstrates Cre-mediated recombination in the neural tube. (A) lacZ expression at 8.5 dpc in the anlage of the diencephalon and mesencephalon. (B) By 9.75 dpc, Bcre32-mediated recombination has targeted the rostral neural tube, including the developing brain and the rostral spinal cord, but largely excluding the telencephalon. (C) lacZ expression throughout the neural tube by 10.5 dpc indicating widespread Bcre32-mediated recombination, except in the dorsomedial telencephalon and regions of the ventral forebrain (detail not shown). (D) At 9.75 dpc, lacZ expression demonstrates Bcre32-mediated recombination throughout the neural ectoderm.





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