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Fig. 10. Integrin adhesion is not required for cell proliferation or establishment
of epithelial polarity. (A) ßPS- clones were induced by
mitotic recombination in the wing disks of
ß
2/ß1 larvae.
Non-GFP expressing cells lack ßPS and ß. Bright green cells are
the progeny of the wild type sibling cell from the mitotic recombination.
Double integrin mutant clones proliferate as well as their twin spots, as seen
by their similar size and cell number. (B,C) ßPS- clones were
induced in the follicular epithelium of
ß2/ß1 female egg
chambers. The apicolateral surfaces of follicle cells are labelled with
antibodies against DE-cadherin, and mutant cells lack GFP. Large clones
lacking GFP are generated, indicating that follicle cell clones lacking both
ß integrin subunits are able to proliferate. (B,B') Surface view of
an egg chamber. DE-cadherin is localized to the cell cortex in double ß
integrin mutant follicle cells. (C,C') Longitudinal confocal section
through an egg chamber. Eliminating integrins from follicle cells causes
multi-layering and cell shape defects. However, in mutant cells that have not
yet completely rounded up, DE-cadherin is properly localized to the
apicolateral surfaces.
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