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Fig. 2. Removal of ß
enhances the defects in midgut morphogenesis caused
by the absence of ßPS. (A,C,E) Longitudinal confocal sections of stage 17
embryos are labelled with phalloidin (inverted, in black), which highlights
the somatic musculature and visceral muscle surrounding the midgut.
Autofluorescence from the yolk is shown in blue. (A) The wild-type midgut is
highly convoluted and is surrounded by a thin layer of visceral muscle. (C)
The midgut forms primary constrictions but fails to elongate in embryos
lacking zygotic ßPS. (E) Midgut constrictions are lost in embryos lacking
zygotic ßPS and maternal and zygotic ß. (B,D,F) High
magnification of visceral muscle labelled with rhodamine phalloidin. (B) The
visceral muscle (vm) in wild type, stage 16 embryos is a single layer of
flattened cells that completely surrounds the columnar midgut epithelium (en,
endoderm). (D) In embryos lacking zygotic ßPS the visceral muscle does
not flatten, but still remains attached to the midgut epithelium. (F) In
embryos lacking both ß subunits, as in E, the visceral muscle is highly
disorganized and detaches from the underlying endoderm.
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