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Fig. 7. ß{nu}-dependent cell-surface expression of {alpha}PS3 indicates that ß{nu} makes a heterodimer with {alpha}PS3. Embryos were labelled with antibodies against the {alpha}PS2 subunit (green) and the {alpha}PS3 subunit (magenta). (A) The {alpha}PS2 subunit is expressed in the somatic and visceral muscles, and localizes to sites of attachment between adjacent muscles in wild-type stage 16 embryos (arrows). The {alpha}PS3 subunit is expressed in the midgut endoderm and is localized cortically (A'). (B) In stage 16 embryos lacking both maternal and zygotic ßPS, {alpha}PS2 failed to be transported to the surface of muscle cells and remained trapped in the endoplasmic reticulum (arrow). However, the plasma membrane expression of {alpha}PS3 was retained in midgut endodermal cells (B'). (C) In the absence of both ß{nu} and zygotic ßPS, the {alpha}PS3 subunit failed to be transported to the surface of endodermal cells (C').





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