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Fig. 2. Sequence alignment of zebrafish psr and its expression profile. (A) A sequence alignment of the PSR protein with the positions of the functional domains shown and indicated by a series of boxes labeled A-D. Box A reveals a potential tyrosine phosphorylation site (from 100-108 aa; KCGEDNDGY), which lies within the predicted intracellular domain (SMART-TMHMM2). The jmjC, A domain (residues 143-206) depicted in box B is part of the cupin metalloenzyme superfamily. In box C (residues 257-287) is an estimate of the secondary structure based upon an assessment of topology (SMART-TMHMM2) and possible hydrophobicity domains. The membrane-associated domain (340-359) contained within box D predicts (SMART-TMHMM2) an extracellular domain that contains the rich supply of serine that potentially may be glycosylated site in this domain. In the block are included all species that are show the complete identity sequences and with the partial identity sequences in all species being shadowed. PSR-H: human PSR; PSR-M: mouse PSR; PSR-F: zebrafish PSR; PSR-D: Drosophila PSR; PSR-C: C. elegans PSR. (B) Expression pattern of PSR from early to late developmental stages probed by in situ antisense RNA hybridization. PSR staining was visualized with a blue color. Topic view of embryos shown in panels a-b, lateral view shown in panels d-f, anterior is to the right side. (a) 512-cell stage, psr is expressed in all cells examined. (b) 30% epiboly, psr is expressed only near the margin of yolk syncytial layer. (c) 2-3 somites, psr are expressed in the whole embryo including the ectoderm, mesoderm and endoderm especially, with the major location for psr being within the brain region (indicated by arrow) and the posterior of the embryo. (d) 24 hpf, psr is expressed principally in the whole notochord (indicated by arrow) from the anterior to the posterior regions, and secondarily distributed in the trunk, brain and the eyes. (e) 36 hpf, psr is expressed similarly to that for 24 hpf but here, psr can be seen to be expressed in the hatching gland and posterior of somite (indicated by arrow). (f) 3 dpf, the psr can be seen to be expressed in some organs such as the heart and the kidney (indicated by arrow). Scale bars denote 100 µm.





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