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Fig. 3. Morpholino-induced knockdown of psr results in the loss of the
cell corpse-engulfment process and cell migration. (A,B) Control-MO-injected
embryos. Lateral (A) and top (B) views of the normal somite (open square and
arrow) reveal the clear furrow of somite at the 6-somites stage (12 hpf). (C)
Lateral view of a PSR-MO-injected embryo reveals abnormal development of the
somite. There is no evidence of the clear furrow and the somite is thinner.
(D) In PSR-MO embryos, a substantial number of cell corpses accumulate in the
somite region (arrows), and an apparent `hole' (long arrow) is formed on the
surface of the somite. Scale bars: 100 µM. (E) In the control-MO group,
complete epiboly and a normal brain (arrow) are observed; the clear bar of the
eye can be seen. (F) In the PSR-MO group, incomplete epiboly (long arrow) and
an abnormal brain (short arrow) are seen; the eye bar is not clearly evident.
Scale bars: 250 µM. (G-J) The sequential pattern of cell corpse
accumulation in whole embryos from 12-36 hpf. Embryonic cells were injected
with control-MO and PSR-MO (40 ng per embryo) at the one-cell stage to block
translation of the psr mRNA. (G,I) Control-MO groups at 12 hpf (G)
and 36 hpf (I); embryos appear normal and no accumulation of cell corpses is
evident. (H,J) PSR-MO groups at 12 hpf (H) and 36 hpf (J); embryos are
abnormal and a gradual accumulation of corpse cells can be seen (arrows).
Scale bars: 250 µM.
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