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Fig. 4. Apoptotic cells identified by Acridine Orange staining and TUNEL assay.
Embryonic cells were injected with control-MO or PSR-MO (40 ng per embryo) at
the one-cell stage to block translation of psr mRNA. (A-C,E) Acridine
Orange staining of 17 hpf control-MO (A), 17 hpf PSR-MO (B), 14 hpf PSR-MO (C)
and 17 hpf PSR-MO (E) embryos. (D) Phase-contrast image of the PSR-MO group at
17 hpf. (F-I) TUNEL assay at 12 hpf stage. (F,H) Phase-contrast images of
control-MO (F) and PSR-MO (H) groups. (G,I) Observation of the embryos under a
fluorescent microscope allows the identification of positive apoptotic cells
in the PSR-MO group (I), when compared with the control-MO group (G). (D,E)
Corpse cells released (see B) accumulate predominantly in the furrow between
somites. AC, apoptotic cells; S, clear somites. Scale bars: 100 µM.
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