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Fig. 6. Morpholino-induced knockdown of psr results in defective brain,
heart and notochord development. Morphological analysis of embryos injected
with 40 ng control (A,C,E,G,I,J) or PSR morpholinos (B,D,F,H,K,L), and
examined at 36 hpf (A,B,E,F) or 3 dpf (C,D,G-I), following staining for
pax2.1 (A,B) or nkx2.5 (E-H), or with Acridine Orange (AO;
I-L), or in the absence of stain (C,D). (A,B) Top view, anterior to the right;
stained with pax2.1. (C,D) Lateral views. The PSR-MO-injected embryo
(B) shows an enlarged brain (long arrow) and an abnormal pax2.1
expression pattern (short arrows), when compared with the control-MO-injected
embryo (A). The enlarged brain includes fore-, mid- and hindbrain morphologies
(indicated by the open square in Fig. 6D; comparative control is shown in C).
(E-L) Investigation of heart development. (E-H) At 36 hpf, the PSR-MO-injected
embryos reveal an absence of normal heart formation (F, arrows and asterisks),
when compared to the atria (A) and ventricles (V) observed in controls (E). By
3 dpf a tube-like heart has formed in the PSR-MO-injected embryos (H,K,L;
arrows), as compared with normal controls (G,I,J). (M-U) The effect of
knockdown of psr on notochord formation at 3 dpf. PSR-MO-injected
embryos (N,P,R,T,U) show abnormal morphological formation such as the bending
of the notochord (N, arrows), when compared with the control-MO group
(M,O,Q,S). Scale bars: 100 µM.
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