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Fig. 1. Colocalization of Isl, Lim3 and Dfr in the embryonic ventral nerve cord. (A) Schematic representation of Isl, Lim3 and Dfr expression in the TN, ISNb and ISNd motoneuron subclasses. (B) Staining for Tau-Myc (red), ß-galactosidase (blue) and Dfr (green) in a stage 16 embryo carrying the isl-tau-myc and Lim3A-lacZ transgenes. Isl, Lim3 and Dfr are co-expressed in the ISNb neurons, including the RP neurons (arrows). Only Isl and Lim3 are expressed in the TN neurons (arrowheads). (C) A stage 16 Lim3A-tau-myc transgenic embryo labeled for Tau-Myc (green) and Dfr (red) expression. Co-expression is seen in the RP neurons (arrow) and the lateral cluster of motoneurons that include MN14/30 and MN 28 (arrowheads). (D) An early stage 15 isl-tau-mycEGFP transgenic embryo labeled for EGFP (green) and Dfr (red) expression. Several lateral EGFP-expressing cells, presumably the ISNd neurons, do not express Dfr (arrows). Dfr and EGFP co-expression is observed in two serotonergic EW neurons at this stage (arrowheads). (E) An abdominal neuromere from a late stage 15 egP289 lacZ enhancer trap line. ß-Gal (green) expression is visible in the three EW neurons and the GW (ISNd) motorneuron. Dfr (red) is not expressed in the GW (ISNd) motoneuron (arrows). (F) Dfr expression (green) is unaffected in an isl37Aa/islDf isl-tau-myc transgenic embryo. The RP (ISNb) motoneurons (arrows) are identified by Myc (red) expression from the transgene. (G) Tau-myc expression (arrows) is not altered in dfrB129/dfrDf Lim3A-tau-myc transgenic embryos.





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