|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 1. (A) The recombinant targeting construct used to generate a mutant mouse
with a disrupted Id4 locus, and a Southern blot and PCR analysis
demonstrating the successful targeting event. (B) E15.5 Id4 mutant
brain (right) and a littermate wild-type control brain (left). (C,C')
Cresyl violet-stained coronal sections through E12.5 wild-type and mutant
telencephalon. Measurements of neocortex and hippocampal primordium shown in
Fig. 2 were made between the
arrows shown. Also, the thickening of the neocortex in the mutant (C')
is indicated by a comparison of the length of the black bar (wild-type
thickness) to the yellow bar (mutant thickness). Basal ganglia (LGE and MGE)
of the mutant appear normal in size. (D,D') E18.5 saggital sections of
control (D) and Id4-/- (D') telencephalon stained
with cresyl violet. (E-H) Quantitation of the cortical VZ surface areas at
E11.5, E12.5 and E15.5, in control and Id4-/- littermates
at matched levels on coronal sections (E,F) and saggital sections (G,H),
normalized to littermate controls. Yellow bar, Id4+/–; blue
bar, Id4-/-; wt, wild type; mt, mutant; LGE, lateral ganglionic
eminence; MGE, medial ganglionic eminence; hp, hippocampus; ctx, neocortex;
OB, olfactory bulb; st, striatum; LV, lateral ventricle.
|
