|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 4. E12.5 (A-C',D') and E15.5 (E-H') coronal sections through
control (A-H) and Id4-/- (A'-H') mutant
cortex. Markers of cell cycle used are: proliferating cells, PCNA
(A,A',D'); S-phase, BrdU pulse (B,B',H,H'); and
M-phase, PH3 (C,C',G,G'). (B,B') The G-phase zone is
indicated by the distance between the arrowheads; yellow dashes indicate the
ventricular surface. (D') High magnification image of PCNA staining
(red), double labeled with DAPI (blue) to visualize all nuclei. At E15.5,
ectopically positioned Ngn2- and NeuroD-expressing cells
were detected outside of the VZ and SVZ (arrowheads in E',F').
Immunofluorescence analysis was performed using antibodies against PH3 and
BrdU (following a 45-minute labelling pulse) in littermate control and
Id4 null animals (arrowheads in G',H' indicate
ectopically proliferating cells). BrdU is shown in green and DAPI in blue
(H,H'). Arrow in H' indicates increased BrdU labeling in the
Id4-/- SVZ at E15.5. LV, lateral ventricle; hp,
hippocampus; ctx, neocortex; LGE, lateral ganglionic eminence; CGE, caudal
ganglionic eminence; VZ, ventricular zone; SVZ, subventricular zone.
|
