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Fig. 7. Mutant rescue and antisense phenocopy confirm that atp2a1 is the acc gene. All panels show video frames 1 second after touch. (A-D) Wild-type SERCA1 mRNA rescues the acc phenotype. Uninjected (A) and acctq206 mRNA-injected (C) acc embryos respond with the aberrant dorsal bend following touch, while acc embryos injected with wild-type SERCA1 mRNA respond normally to touch (B). This embryo coiled twice and relaxed 1 second after touching. Injection of SERCA1 mRNA carrying a cognate Brody disease mutation does not rescue the acc phenotype (D). (E-H) Antisense atp2a1/SERCA1 phenocopies the acc phenotype. Antisense MO-injected wild-type embryos respond to touch with the dorsal bend (F), while control MO-injected embryos respond with coils and are relaxed 1 second after touching (E). Co-injection of wild-type SERCA1 mRNA with antisense MO blocks the induction of the mutant phenotype and shows the normal coils and relaxation to touch (G), whereas co-injection with mutant mRNA does not (H).





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