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Fig. 5. Transcriptional activation by LEF1 in response to Wnt1 signaling in
primary-cultured fibroblasts from newborns. Fibroblasts of rs5 (+/+), rs7
(rs/+), rs3 (rs/rs) and rs8 (rs/rs) were
transfected with a LEF-LUC reporter construct (500 ng), in combination with
expression plasmids encoding LEF1 (50 ng), ß-catenin (300 ng) or Wnt1
(300 or 1000 ng). The levels of luciferase activity were normalized for the
expression of a co-transfected Rous sarcoma virus-ß-galactosidase
expression plasmid (50 ng). The normalized activity was quantitated relative
to the level of activity (100%) from cells transfected with LEF1 and
ß-catenin expression plasmids. Means with error bars representing
standard errors. Experiments were repeated at least three times.
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