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Fig. 3. Thick cells, but not round cells, express myogenin. Myofibers obtained from
gastrocnemius muscle were cultured in pmGM for 7 days. Then, satellite
cell-derived colonies were subjected to immunostaining with anti-myogenin
antibody. (A,B) A fraction of thick cells (arrowheads) around a myotube
(asterisk) expressed myogenin (green in B). Scale bar: 50 µm. (C,D) One of
the thick cells (arrowhead), but none of the round cells (within the square),
expressed myogenin (D). Scale bar: 20 µm. Nuclei were visualized with DAPI
(blue in B and D). (A,C) Phase contrast microscopy images of the same fields
as those shown in B and D.
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