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Fig. 5. Basic FGF and LIF synergistically suppress the expression of MHC. (A-D)
Single myofibers isolated from gastrocnemius muscle were cultured in pmGM (A),
or in pmGM supplemented with bFGF alone (B), LIF alone (C), or bFGF plus LIF
(D), for 6 days. Cells were then subjected to immunostaining for MHC (red).
Nuclei were visualized by DAPI. Scale bars: 100 µm. (E) Quantitative
analysis of inhibition of MHC expression by growth factors. Percentages of
nuclei in MHC-expressing cells to total nuclei (bars) and total numbers of
nuclei in each field (0.55 mm2) (open circles) were calculated in
four independent samples. Averages and standard errors are shown.
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