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Fig. 2. Overexpression of Dx ectopically activates the target genes of N signaling.
(A-C) Within the Dx-overexpressing clones (green), vgBE (purple) was activated
in a cell autonomous manner. C is a merged image of A and B. (D-F) Within
cells overexpressing Dx (green), the wg promoter was ectopically
activated. The promoter activity of wg was detected by
wg-lacZ (purple). F is a merged image of D and E. (G-I) Within cells
overexpressing Dx (green), the Dl promoter was ectopically activated.
The promoter activity of Dl was detected by Dl-lacZ
(purple). I is a merged image of G and H. Weak cell non-autonomous induction
of Dl-lacZ was also observed. (J,L) Expression of N in wild-type wing
discs. N was detected by an anti-NICD antibody (green). (K,L)
Activation of the N promoter was visualized by N-lacZ
(purple). (M-O) Within the Dx-overexpressing clones (green), N-lacZ
(purple) was activated in a cell autonomous manner. O is a merged image of M
and N. (P-R) Within the NICD-overexpressing clones (green),
N-lacZ (purple) was activated cell autonomously. R is a merged image
of P and Q. All images were obtained from third-instar wing discs with
respective genetic manipulation.
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