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Fig. 6. Dx co-localizes with N in the late-endosomes. (A-L) Confocal microscopic
images of third-instar wing discs overexpressing Dx. (A-D) Some of the Dx
(blue in C and D) immunoreactivity co-localized with Clc-GFP (green in A and
D) and N (purple in B and D). D is a merged image of A, B and C. (E-H)
Drosophila Hk exists exclusively in the early-endosomes. Neither N
(green in E and H) nor Dx (blue in G and H) co-localized with Hk (purple in F
and H). H is a merged image of E, F and G. (I-L) Drosophila Rab7-GFP
accumulates in the late-endosomal compartment. Dx (blue in K and L) and
endogenous N (purple in J and L) co-localize in the late-endosome (green in I
and L). L is a merged image of I, J and K. (M-P) A fusion protein of Dx and
YFP (green in M, O and P) was expressed in the Drosophila S2 cell
line. The lysosomes were visualized by LysoTracker (purple in N, O and P). O
is a merged image of M and N. P is a merged picture of the optical microscopic
image of a cell with O. (A-L) UAS-dx was driven by ptc-GAL4.
(M-P) UAS-YFP-dx was driven by pWA-GAL4.
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