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Fig. 10. Lack of the optic vesicles and the ganglionic eminences in
Hes1;Hes5 double-mutant brain. (A,B) SEM analysis shows the optic
vesicle (arrow) and the ganglionic eminence (arrowhead) in the wild type (A)
while such structures are not formed in the Hes1;Hes5 double-mutant
brain (B). In some regions, cell arrangement is disrupted (B, arrows). (C-G)
Immunohistochemistry for TuJ1. Neurons (TuJ1+, green) are not
generated in the wild type optic vesicle at E9.5 (C) and E10.5 (E,F). By
contrast, in Hes1;Hes5 double-mutants, neurons are already
differentiated at E9.5 (D) and E10.5 (G) in the region that should normally
become optic vesicles. The nuclei are counterstained with PI (red). (H,I) HE
staining of the wild type (H) and Hes1;Hes5 double mutant (I). In the
double mutant, the eye is not formed (I, arrow). (J) Rx is expressed
similarly in both the wild type and Hes1;Hes5 double-mutant. (K-N)
Pax2 and Chx10 are expressed in the wild-type eye (K,M) but
not in the double-mutant (L,N). Scale bars: 500 µm in A,B; 100 µm in
C-E,G; 200 µm in H,I.
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