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Fig. 4. Premature loss of radial glia in Hes-mutant spinal cord. Histological analysis was done with the wild-type (A-C,G-I) and Hes1;Hes5 double-mutant (D-F,J-L). The dorsal is up in all panels. At E8.5, the neural plate consists of nestin⁺ neuroepithelial cells in Hes1;Hes5 double-mutant (D) as in the wild type (A). At E9.5 and E10.5, nestin⁺RC2⁺ radial glial cells are present throughout the wall of the spinal cord in the wild type (B,C,H,I). By contrast, in the double mutant, nestin⁺RC2⁺ radial glial cells are prematurely lost from the ventral region (E,F,K,L). Scale bars: 100 µm.

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