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Fig. 5. Premature neurogenesis in Hes1;Hes5 double mutants. (A) In the wild type, radial glial cells (Ki67+) are located throughout the wall while neurons (TuJ1+) reside in the outer layer at E10.5. (E) In Hes1;Hes5 double mutants, there are numerous neurons (TuJ1+) while radial glial cells (Ki67+) are significantly decreased. (B-D,F-H) In-situ hybridization analysis shows that expression of Dll1, Mash1 and Math3 is highly upregulated in Hes1;Hes5 double mutants (F-H), compared with the wild type (B-D). Scale bar: 100 µm in A,E.





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