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Fig. 2. Deletion of distal cranial sensory ganglia in
Eya1–/– and
Six1–/– embryos. (A-C) Whole-mount
hybridization with an SCG10 riboprobe of wild-type (A),
Eya1–/– (B), and
Six1–/– (C) embryos at E10.5. A lack of
neuronal differentiation of VIIIth and distal VIIth, IXth and Xth precursors
is observed in Eya1–/– embryos. In
Six1–/– embryos, SCG10 staining was
also missing in the regions of VIIIth and distal VIIth ganglia and slightly
reduced in the regions of distal IXth and Xth ganglia. (D-F) E9.5 embryos
hybridized with an Neurog2 riboprobe. Neurog2 expression in
distal VII, IX and X placodes is readily visible in wild-type embryos, but its
expression is completely lost in these structures in
Eya1–/– embryos. In
Six1–/– embryos, Neurog2 expression
is undetectable in the VIIth placode and markedly reduced in the IXth and
reduced in Xth placode. (G-O) Section in situ hybridization with the
Neurog2 riboprobe of wild-type (G,J,M),
Eya1–/– H,K,N) and
Six1–/– (I,L,O) embryos at E8.5 to 9.25.
Strong Neurog2 expression was observed in the distal VIIth placode
(VIIp), and some migratory precursors (arrowhead) at E8.5-9.0 and in the
distal IXth placode (IXp), and migratory precursors (arrowhead) at E8.75-9.25
and the Xth placode (Xp) at E9.0-9.25 in wild-type embryos, whereas its
expression is completely absent in these structures of
Eya1–/– at these stages (arrow, H,K,N). In
Six1–/– embryos, its expression was reduced to
background level in the VIIth precursors (arrow, I), also slightly reduced in
the IXth (arrow, L) and relatively normal in the Xth precursors (arrow,
O).
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