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Fig. 3. Alteration of Neurog1, Neurod, Math3 and Nscl1 expression
in Eya1–/– and
Six1–/– epibranchial neuronal precursors.
(A-F) Transverse section hybridization showing Neurog1 expression in
the otic vesicle (ov) and in the VIIIth ganglion region and epibranchial (VII,
IX) neuronal precursors in wild-type embryos (A,D), no expression in
Eya1–/– epibranchial neuronal precursors
(arrow in B,E) and reduced expression in
Six1–/– epibranchial neurons (arrow in C,F).
Note that Neurog1 expression was observed in the VIIIth precursors
(open arrow in B,C). (G-L) Transverse section hybridization showing
Neurod expression in the VIIIth and epibranchial neuronal precursors
in wild-type embryos (G,J), no expression in epibranchial precursors in
Eya1–/– embryos (arrow in H,K) and no
expression (arrow in I) or reduced expression in
Six1–/– epibranchial neurons (arrow in L).
Note that Neurod expression was detected in the VIIIth neuroblast
precursors (open arrow in H,I). (M-O) Whole-mount hybridization showing
Math3 expression in epibranchial neurons in wild-type embryos (M),
only a few Math3-positive cells in
Eya1–/– Xth neurons (open arrow in N), and
reduced expression in Six1–/– IXth and Xth
neurons (O). (P-R) Whole-mount hybridization showing Nscl1 expression
in cranial sensory ganglia in wild-type embryos (P). In
Eya1–/– embryos, while Nscl1
expression appeared relatively normal in the Vth ganglion, it expression is
absent in VIIIth, VIIth and IXth ganglia and very few Nscl1-positive
cells were detected in Eya1–/– Xth neurons
(open arrow in Q). (R) Nscl1 expression is also absent in
Six1–/– VIIIth ganglion and markedly reduced
in Six1–/– VIIth (arrowhead) and IXth (arrow)
ganglia.
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