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Fig. 5. Malformation of cranial sensory ganglia in
Eya1–/– or
Six1–/– embryos at later stages. (A-F)
Neurofilament antibody staining of sagittal sections of E11.5 wild-type (A,D),
Eya1–/– (B,E) and
Six1–/– (C,F) heads. Neurofilament antibody
labeled the proximal VIIth ganglion (pVII), which is normally fused with the
VIIIth ganglion (gVIII), the proximal IXth (pIX) and Xth (pX) and the distal
VIIth (gVII), IXth (gIX) and Xth (gX) ganglia in wild-type embryos (A,D). In
Eya1–/– embryos, all proximal ganglia and
partial structures of the distal Xth ganglion are present, but the VIIIth and
the distal VIIth and IXth ganglia are missing (B,E). (C,F) In all three
Six1–/– embryos, while the proximal ganglia
are present, the VIIIth and distal VIIth (gVII) ganglia are missing but the
distal IXth and Xth ganglia are also present. For A-F, anterior is to the left
and dorsal is up. (G-I) Hematoxylin and Eosin and (J-L) neurofilament antibody
staining of transverse sections of E12.5 wild-type (G,J),
Eya1–/– (H,K) and
Six1–/– (I,L) heads. (G,J) The IXth ganglion
(gIX) and the nerve fibers near the IXth ganglion that strongly stained with
both Eosin and the neurofilament antibody are present in wild-type embryos
(arrow). The neurofilament antibody also strongly labeled other nerve fibers
in that region, including the VIIth nerve fibers (nVII, J). (G,K) The IXth
ganglion is missing in Eya1–/– embryos.
However, some nerve fibers that strongly stained with both Eosin and the
neurofilament antibody are present in the mutant and this structure is likely
to represent the Xth nerve fibers, which are present at E11.5 (E). (I,L) The
IXth ganglion is present in Six1–/– embryos
with slightly smaller size and the neurofilament antibody also labeled nearby
nerve fibers (arrow and nX/XI). For G-L, dorsal is up and lateral is to the
left. jv, internal jugular vein; m, prevertebral premuscle mass; sg,
sympathetic ganglion component.
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