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Fig. 3. Hh signaling is required for Bar expression in the basal undifferentiated cells. Eye discs containing smo3 LOF clones at different positions, close to the posterior margin (A-D) and near the furrow (E-H), are stained with antibodies for lacZ (clone marker; red), BarH1 (green) and Ato (blue). Bar expression is lost in smo3 LOF clones located close to the posterior margin (C, white arrow) or near the furrow (G, white arrow). Some Bar expression remains in part within the smo3 LOF clone near wild-type cells (C,G; yellow arrows), and ectopic Ato expression is observed in the region of Bar loss within the clones (D,H; red arrows). Apical through basal sections of confocal images are combined in order to see the BarH1 expression in single image.





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