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Fig. 3. Effects of irradiation and heat shock in wing disc development. (A-C)
Untreated disc and adult wing of genotype hh-Gal4>UAS-p35 UAS-GFP.
It shows normal wg expression (red) and a normal P compartment
(green). The expression of wg is not affected by P35 and the
differentiated wing in C is essentially normal, except a slight shortening of
vein 5. (D-F) Disc and adult wing from larvae of the same genotype, but
irradiated in the first larval period. Note the abnormal shape and bigger size
of the P compartment (green) and the numerous foci of ectopic and elevated
wg expression. wg expression in the A compartment remains
normal. The antero-posterior demarcation line is highly irregular and there
are indentations into the A compartment (arrow). The few flies that hatch
after this early treatment differentiate wings with very abnormal and
overgrown P compartments (F). (G-H) Disc of the same genotype dissected from a
larva heat shocked at the first larval period. Note the appearance of numerous
dots of ectopic wg expression in the P compartment and the protrusion
into the A compartment (arrow). (I-J) Irradiated disc showing ectopic
dpp expression in the P compartment. An arrow points to an
indentation into the A compartment
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