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Fig. 4. Endoneurial fibroblasts are neural crest derived. Sciatic nerves were
dissected from P11 Wnt1-Cre+loxpRosa+ pups and processed
for immunohistochemistry (A,B), or electron microscopy (C-H). Nerves were
stained with antibodies against ß-gal (red) to identify neural
crest-derived cells, and S100ß (green) to identify Schwann cells. The
same field of view is shown in A and B, with DAPI staining (blue) added to
panel B. In addition to ß-gal+S100ß+ glia
(green or yellow), we also observed
ß-gal+S100ß– endoneurial cells (white
arrows) that were neither associated with axons nor blood vessels. By electron
microscopy, we consistently observed endoneurial fibroblasts stained with
bluo-gal, indicating neural crest-derivation (C-F). (C,D) Bluo-gal-stained
endoneurial fibroblasts (f), surrounded by myelinating Schwann cells, most of
which are also stained with bluo-gal. Red arrows indicate bluo-gal crystals in
the fibroblast; green arrows indicate bluo-gal crystals in Schwann cells. (E)
A lower magnification image of a large bluo-gal stained fibroblast, with a
very long process, surrounded by Schwann cells. The boxed area is shown at a
higher magnification in F. Note the presence of a basal lamina around the
Schwann cells (black arrows), but the lack of a basal lamina around the
fibroblast process. (G,H) Low and high power images of sections through
control littermates that lack any bluo-gal staining in perineurial cells (G),
Schwann cells (G,H) or fibroblasts (H).
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