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Fig. 5. Dhh-Cre expressing progenitors give rise to endoneurial fibroblasts as well
as Schwann cells in nerves. Sciatic nerves were dissected from P11
Dhh-Cre+LoxpRosa+ (A,B) pups as well as
Dhh-Cre–LoxpRosa+ (C) littermate controls,
processed for bluo-gal staining, and examined by electron microscopy.
Endoneurial fibroblasts (f) were identified by their angular shape, long
processes (A), and lack of a basal lamina (B). Red arrows indicate bluo-gal
crystals in an endoneurial fibroblast; green arrows indicate bluo-gal crystals
in Schwann cells. B shows the inset from panel A at a higher magnification to
demonstrate the presence of a basal lamina on Schwann cells (black arrows),
and the absence of a basal lamina on the fibroblast. Bluo-gal crystals were
never observed in littermate control nerves (C). Endoneurial fibroblasts
expressed ß-gal at a similar frequency to Schwann cells, whereas
perineurial cells were nearly all negative (D).
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