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Fig. 2. Expression patterns of XPIASy and XSmad2 during
Xenopus development. (A) Semi-quantitative RT-PCR analysis of
XPIASy and XSmad2 expression was performed using whole
embryos at different stages. ODC (Ornithine decarboxylase)
was used as a control. (B) XPIASy and XSmad2 show different
temporal expression in the marginal zone at the early gastrula stage. Stage 10
embryos were dissected as shown into animal pole (AN), vegetal pole (VG), DMZ
(D) or VMZ (V) explants, or whole embryos (WE), and subjected to RT-PCR
analysis of XPIASy and XSmad2. XPIASy is expressed strongly
in the VMZ compared with the DMZ. (C) In situ hybridization of XPIASy
(a-g) and XSmad2 (h-n) during development. (a,h) Stage 6-1/2, lateral
view; (b,i) stage 10; (c,j) stage 18, anterior view; (d,k) stage 25; (e,l)
stage 27; (f,m) stage 29/30; and (g,n) stage 37/38.