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Fig. 1. The –8.4ngn1:gfp zebrafish transgene recapitulates the
pattern of endogenous ngn1 transcripts and drives telencephalic
expression in the zebrafish embryo. (A) The 8.4 kb upstream zebrafish
ngn1 regulatory sequence drives GFP expression in the telencephalon
(arrow) and in the diencephalon. (B,C) Comparison of the endogenous
ngn1 gene (B) and the gfp transgene (C) by in situ
hybridisation indicates that the gfp transgene is capable of
recapitulating the endogenous telencephalic (t, indicated by arrows) and
diencephalic expression of ngn1. Expression is detected in at least
six regions of the diencephalon, comprising the epiphysis (e), pretectum
(pre), dorsal thalamus (dt), ventral thalamus (vt), preoptic area (po) and
posterior tuberculum (pt). Transcripts are also localised in the midbrain
tegmentum (tg). Zebrafish embryos are at stage 28 hpf. Panels show lateral
views of whole-mounted embryos oriented anterior to the left and dorsal
up.
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