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Fig. 4. The LATE region is required for expression in the diencephalon and
hindbrain of zebrafish embryos. (A) The –3.1ngn1:gfp transgene,
harbouring LATE, drives gfp expression extensively in the
diencephalon and in the hindbrain. This pattern is partially reminiscent of
the expression of the two zebrafish pax6 genes (see
Fig. 5D,E). (B) Upon deletion
of LATE in –3.1(delLATE)ngn1:gfp transgenic embryos,
expression of gfp mRNA in the diencephalon and in the hindbrain is
strongly reduced. (C,D) Deletion of LATE in the
–8.4ngn1:gfp transgenics reduced reporter expression in the
diencephalon (arrowhead) but not in the telencephalon (t). Thus, the activity
of LATE is required for transgene expression in the diencephalon.
Embryos were oriented with anterior directed towards the left and dorsal
up.
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