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Fig. 8. Pax6 binds to site-C of the LATE region. Electromobility-shift
assays with recombinant mouse Pax6 protein. Radioactively labelled
oligonucleotide containing site-C of the LATE region was incubated
with Pax6 protein without competitor oligonucleotide, or with a 50-fold molar
excess of cold site-C oligonucleotide (sC), or with an oligonucleotide that
contained a cluster of point mutations (msC) in the Pax6-binding site homology
domain. The site-C competitor abolished the shifted band, whereas the complex
formation was not affected by the presence of the mutated oligonucleotide,
demonstrating that the interaction of Pax6 protein is dependent on an intact
Pax6-binding site. As a positive control, an oligonucletide (Control)
harbouring the Pax6-binding site described by Czerny et al.
(Czerny et al., 1993) was
used.
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